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Beef consumption increases every year in Indonesia. However, beef must be considered safe and halal. Complete bleeding during slaughter will cause the quality of the meat to be good. One way to determine this is to test the blood content in beef. In general, meat is obtained from the process with or without stunning before slaughter. This study compares the potential of a smartphone camera with a spectrophotometer to measure the blood content in beef with and without stunning before slaughter at the slaughterhouse and at the site of slaughter of sacrificial animals. This study used 12 local cattle for each treatment. The green solution of 0.1% beef extract and Malachite Green was reacted with the addition of H₂O₂ solution. If there is no hemoglobin in the meat extract, the mixed solution changes color gradually from green to blue according to the hemoglobin content in the meat extract. The gradation of this color change was measured using a spectrophotometer as the gold standard and the image processing technique produced by a smartphone camera using ImageJ software. The results obtained were converted into concentrations using the Lambert-Bier formula. The blood concentration in beef obtained by stunning was lower than that obtained without stunning before slaughtering using either spectrophotometer A, spectrophotometer B, or smartphone camera devices but not significantly different after T test using SPSS (P>0.05). The correlation test of the tool produces a regression equation y=1.161x-0.000 between spectrophotometers A and B and y=0.5613x-0.0005 between spectrophotometer A and camera devices with correlation coefficients of 0.9962 (very strong) and 0.7431 (strong) so that mobile cameras have the potential to measures the concentration of blood in meat.