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Chronic Kidney Disease (CKD) is an irreversible decline in kidney function that can be fatal without hemodialysis, urea and creatinine level was useful biomarker for monitoring kidney damage. Most of CKD patient has to undergo hemodialysis for a lifetime, while hemodialysis has some complication that could affected the patient’s adherence to the treatment that may caused escalation on their urea and creatinine levels. The purpose of this study was to determine the correlation between length of hemodialysis with creatinine and urea  levels. This research is a non reactive research based on medical record of CKD patients on hospital that has a hemodialysis unit in Kupang City, the study population was all hemodialysis patients total 117 peoples, this study involved 93 patients using purposive sampling technique. Data analysed using Spearman correlation. The results of this study are lenght of hemodialysis and serum urea levels (p=0.150 > 0.05) and lenght of hemodialysis and serum creatinine levels (p=0.0600 >0.05), findings of this study were length of hemodialysis mean was 22.45 months, most of CKD patient has abnormal urea and creatinine levels (98.9% and 97.8%, respectively). There are other factors could affected urea and creatinine levels besides length of hemodialysis. Conclusion no significant correlation between the length of hemodialysis with serum urea and serum creatinine levels on CKD patients.